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real time fluorometers  (Hologic Inc)

 
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    Hologic Inc real time fluorometers
    Real Time Fluorometers, supplied by Hologic Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/real+time+fluorometers/us12655486-777-16-18?v=Hologic+Inc
    Average 86 stars, based on 1 article reviews
    real time fluorometers - by Bioz Stars, 2026-06
    86/100 stars

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    Image Search Results


    (A) A representative image of real-time RT-RPA amplification products using the Twista fluorometer. The graph depicts the fluorescent signal for each template used in the RT-RPA reactions and the key to the left indicates the template each color represents. At approximately five minutes into run time a required Magnesium acetate (280 nM) adding step, to initiate the RPA reaction, was performed resulting in signal not being generated for the duration of that step (gap in the graph). (B) RPA amplification of Dengue 2 M gene. The numbers in the left margin indicate selected bands of the DNA marker, and the estimated amplified product size is indicated at 316 bp in the right margin. Lane 1 is a 50bp DNA ladder (Thermo Scientific™), lane 2 is the no-template control (water as template). Lanes 3 represent RPA amplification product from 10 copies of DENV-2 genome per reaction at 37°C.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Development of a rapid point-of-care dengue virus type 2 infection diagnostic assay using recombinase polymerase amplification and lateral flow device

    doi: 10.3389/fcimb.2025.1578549

    Figure Lengend Snippet: (A) A representative image of real-time RT-RPA amplification products using the Twista fluorometer. The graph depicts the fluorescent signal for each template used in the RT-RPA reactions and the key to the left indicates the template each color represents. At approximately five minutes into run time a required Magnesium acetate (280 nM) adding step, to initiate the RPA reaction, was performed resulting in signal not being generated for the duration of that step (gap in the graph). (B) RPA amplification of Dengue 2 M gene. The numbers in the left margin indicate selected bands of the DNA marker, and the estimated amplified product size is indicated at 316 bp in the right margin. Lane 1 is a 50bp DNA ladder (Thermo Scientific™), lane 2 is the no-template control (water as template). Lanes 3 represent RPA amplification product from 10 copies of DENV-2 genome per reaction at 37°C.

    Article Snippet: Amplification was monitored using the portable Twista real-time fluorometer (TwistDx) ( ).

    Techniques: Amplification, Generated, Marker, Control

    Combined Probit regression plot comparing Twista (fluorescence detection) and LFD (lateral flow): • Solid blue curve: Twista with 95% CI • Solid Orange curve: LFD with 95% CI • Blue circles: Observed Twista results • Orange cross: Observed LFD results • Dashed vertical lines show the estimated LoD at 95% detection for each method: o Twista ≈ 38.48 copies/reaction o LFD ≈ 50.37 copies/reaction.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Development of a rapid point-of-care dengue virus type 2 infection diagnostic assay using recombinase polymerase amplification and lateral flow device

    doi: 10.3389/fcimb.2025.1578549

    Figure Lengend Snippet: Combined Probit regression plot comparing Twista (fluorescence detection) and LFD (lateral flow): • Solid blue curve: Twista with 95% CI • Solid Orange curve: LFD with 95% CI • Blue circles: Observed Twista results • Orange cross: Observed LFD results • Dashed vertical lines show the estimated LoD at 95% detection for each method: o Twista ≈ 38.48 copies/reaction o LFD ≈ 50.37 copies/reaction.

    Article Snippet: Amplification was monitored using the portable Twista real-time fluorometer (TwistDx) ( ).

    Techniques: Fluorescence